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lotus tetragonolobus lectin ltl  (Vector Laboratories)


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    Structured Review

    Vector Laboratories lotus tetragonolobus lectin ltl
    Lotus Tetragonolobus Lectin Ltl, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 612 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/FL-1321-2/Fluorescein+labeled+Lotus+Tetragonolobus+Lectin+(LTL)/pm42019644-89-0-6
    Average 96 stars, based on 612 article reviews
    lotus tetragonolobus lectin ltl - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Labeling:

    Article Title: Geometrically Constrained Growth Factor Concentration Favors Enrichment of Goblet Cells and Mucus Formation.
    Article Snippet: Other primary antibodies staining AGR2 (Invitrogen, PA5-34517, 1:200 dilution), RELM-β (Invitrogen, PA561896, 1:200 dilution), and TFF3 (Invitrogen, PA5-57279, 1:200 dilution) were detected by the secondary antibody goat antirabbit IgG conjugated to Alexa Fluor 594 (Jackson ImmunoResearch, 111-585- 003) diluted to 1 μg/mL in 1% BSA in PBS with 1 μg/mL Hoechst 33342 and incubated with the cells for 1 h at 22 °C. .. Fluorescently labeled lectins, Lycopersicon esculentum lectin (LEL, Vector Lab, DL1177, 1:100 dilution), Lotus tetragonolobus lectin (LTL, Vector Lab, FL-1321, 1:200 dilution), and wheat germ agglutinin (WGA, Invitrogen, W11261, 1:100 dilution) lectin were used to label carbohydrate groups on mucins. .. A labeled primary antibody against mucin 1 (Muc1, Invitrogen, sc-7313 AF594, 1:100 dilution) was diluted in IF with 1 μg/mL Hoechst 33342 and incubated with the cells for 24 h at 4 °C.

    Whole Genome Amplification:

    Article Title: Geometrically Constrained Growth Factor Concentration Favors Enrichment of Goblet Cells and Mucus Formation.
    Article Snippet: Other primary antibodies staining AGR2 (Invitrogen, PA5-34517, 1:200 dilution), RELM-β (Invitrogen, PA561896, 1:200 dilution), and TFF3 (Invitrogen, PA5-57279, 1:200 dilution) were detected by the secondary antibody goat antirabbit IgG conjugated to Alexa Fluor 594 (Jackson ImmunoResearch, 111-585- 003) diluted to 1 μg/mL in 1% BSA in PBS with 1 μg/mL Hoechst 33342 and incubated with the cells for 1 h at 22 °C. .. Fluorescently labeled lectins, Lycopersicon esculentum lectin (LEL, Vector Lab, DL1177, 1:100 dilution), Lotus tetragonolobus lectin (LTL, Vector Lab, FL-1321, 1:200 dilution), and wheat germ agglutinin (WGA, Invitrogen, W11261, 1:100 dilution) lectin were used to label carbohydrate groups on mucins. .. A labeled primary antibody against mucin 1 (Muc1, Invitrogen, sc-7313 AF594, 1:100 dilution) was diluted in IF with 1 μg/mL Hoechst 33342 and incubated with the cells for 24 h at 4 °C.

    Marker:

    Article Title: NUPR1 alleviates renal ischemia reperfusion injury by inhibiting ferroptosis involving the ATF4-CHAC1 pathway.
    Article Snippet: As a major cause of acute kidney injury (AKI), renal ischemia reperfusion injury (IRI) poses a considerable clinical challenge worldwide.. Currently, effective therapeutic strategies for renal IRI remain limited.. Although ferroptosis has been identified as a pivotal factor in the pathogenesis of renal IRI, the molecular mechanisms underlying the regulation of ferroptosis in renal IRI require further elucidation.

    Article Title: TMEM174 Deficiency Reduces Longevity by Promoting Phosphate-Driven Vascular Calcification
    Article Snippet: Immunostaining analysis of mouse kidney sections (Zyagen) was performed as described previously . .. For immunofluorescence, the section was incubated with affinity-purified anti-TMEM174 monoclonal antibody (Biomatik, this antibody was generated with a full-length mouse TMEM174 protein as the antigen), anti-mouse NPT2A polyclonal antibody and Lotus Tetragonolobus Lectin (LTL, a renal apical membrane marker, Vector Laboratories FL-1321-2) overnight at 4°C. .. Immunoreactivity was detected using Alexa Fluor 488-conjugated anti-mouse IgG (Thermo Fisher Scientific A-11001) or Alexa Fluor 568-conjugated anti-rabbit IgG (Thermo Fisher Scientific A-11011).

    Staining:

    Article Title: NUPR1 alleviates renal ischemia reperfusion injury by inhibiting ferroptosis involving the ATF4-CHAC1 pathway.
    Article Snippet: As a major cause of acute kidney injury (AKI), renal ischemia reperfusion injury (IRI) poses a considerable clinical challenge worldwide.. Currently, effective therapeutic strategies for renal IRI remain limited.. Although ferroptosis has been identified as a pivotal factor in the pathogenesis of renal IRI, the molecular mechanisms underlying the regulation of ferroptosis in renal IRI require further elucidation.

    Immunofluorescence:

    Article Title: TMEM174 Deficiency Reduces Longevity by Promoting Phosphate-Driven Vascular Calcification
    Article Snippet: Immunostaining analysis of mouse kidney sections (Zyagen) was performed as described previously . .. For immunofluorescence, the section was incubated with affinity-purified anti-TMEM174 monoclonal antibody (Biomatik, this antibody was generated with a full-length mouse TMEM174 protein as the antigen), anti-mouse NPT2A polyclonal antibody and Lotus Tetragonolobus Lectin (LTL, a renal apical membrane marker, Vector Laboratories FL-1321-2) overnight at 4°C. .. Immunoreactivity was detected using Alexa Fluor 488-conjugated anti-mouse IgG (Thermo Fisher Scientific A-11001) or Alexa Fluor 568-conjugated anti-rabbit IgG (Thermo Fisher Scientific A-11011).

    Incubation:

    Article Title: TMEM174 Deficiency Reduces Longevity by Promoting Phosphate-Driven Vascular Calcification
    Article Snippet: Immunostaining analysis of mouse kidney sections (Zyagen) was performed as described previously . .. For immunofluorescence, the section was incubated with affinity-purified anti-TMEM174 monoclonal antibody (Biomatik, this antibody was generated with a full-length mouse TMEM174 protein as the antigen), anti-mouse NPT2A polyclonal antibody and Lotus Tetragonolobus Lectin (LTL, a renal apical membrane marker, Vector Laboratories FL-1321-2) overnight at 4°C. .. Immunoreactivity was detected using Alexa Fluor 488-conjugated anti-mouse IgG (Thermo Fisher Scientific A-11001) or Alexa Fluor 568-conjugated anti-rabbit IgG (Thermo Fisher Scientific A-11011).

    Affinity Purification:

    Article Title: TMEM174 Deficiency Reduces Longevity by Promoting Phosphate-Driven Vascular Calcification
    Article Snippet: Immunostaining analysis of mouse kidney sections (Zyagen) was performed as described previously . .. For immunofluorescence, the section was incubated with affinity-purified anti-TMEM174 monoclonal antibody (Biomatik, this antibody was generated with a full-length mouse TMEM174 protein as the antigen), anti-mouse NPT2A polyclonal antibody and Lotus Tetragonolobus Lectin (LTL, a renal apical membrane marker, Vector Laboratories FL-1321-2) overnight at 4°C. .. Immunoreactivity was detected using Alexa Fluor 488-conjugated anti-mouse IgG (Thermo Fisher Scientific A-11001) or Alexa Fluor 568-conjugated anti-rabbit IgG (Thermo Fisher Scientific A-11011).

    Generated:

    Article Title: TMEM174 Deficiency Reduces Longevity by Promoting Phosphate-Driven Vascular Calcification
    Article Snippet: Immunostaining analysis of mouse kidney sections (Zyagen) was performed as described previously . .. For immunofluorescence, the section was incubated with affinity-purified anti-TMEM174 monoclonal antibody (Biomatik, this antibody was generated with a full-length mouse TMEM174 protein as the antigen), anti-mouse NPT2A polyclonal antibody and Lotus Tetragonolobus Lectin (LTL, a renal apical membrane marker, Vector Laboratories FL-1321-2) overnight at 4°C. .. Immunoreactivity was detected using Alexa Fluor 488-conjugated anti-mouse IgG (Thermo Fisher Scientific A-11001) or Alexa Fluor 568-conjugated anti-rabbit IgG (Thermo Fisher Scientific A-11011).

    Membrane:

    Article Title: TMEM174 Deficiency Reduces Longevity by Promoting Phosphate-Driven Vascular Calcification
    Article Snippet: Immunostaining analysis of mouse kidney sections (Zyagen) was performed as described previously . .. For immunofluorescence, the section was incubated with affinity-purified anti-TMEM174 monoclonal antibody (Biomatik, this antibody was generated with a full-length mouse TMEM174 protein as the antigen), anti-mouse NPT2A polyclonal antibody and Lotus Tetragonolobus Lectin (LTL, a renal apical membrane marker, Vector Laboratories FL-1321-2) overnight at 4°C. .. Immunoreactivity was detected using Alexa Fluor 488-conjugated anti-mouse IgG (Thermo Fisher Scientific A-11001) or Alexa Fluor 568-conjugated anti-rabbit IgG (Thermo Fisher Scientific A-11011).



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    SCAP/SREBPs upregulation induces lipid deposition and inflammation in DN. ( A ) Multispecies conjoint analysis of conserved biological process in DN. ( B ) Schematic of SCAP/SREBPs as integrators of lipid metabolic and inflammatory signaling pathways. ( C , D ) Representative immunohistochemical staining for SREBP1, SREBP2, and SCAP in renal glomeruli ( C ) and tubules ( D ) of DN patients ( n = 6). Scale bars = 20–50 μm. ( E ) Representative immunohistochemical staining of CD68 in kidney of DN patients ( n = 6). Scale bar = 50 μm. ( F ) Heat map of Scap, Srebf1, Srebf2 and their downstream target gene expression in DN mice ( n = 6) from GSE200322 . ( G ) Correlation analysis between Scap, Srebf1, Srebf2 and renal inflammation, fibrosis, glomerular/tubular injury markers in DN mice ( n = 6) from GSE200322 . ( H ) Scheme of the STZ/HFD-induced T2DN mouse model. ( I ) Representative Oil Red O staining of renal glomeruli and tubules in DN mice ( n = 6). Scale bar = 10–50 μm. ( J , K ) Representative immunohistochemical staining for SREBP1, SREBP2, and SCAP in renal glomeruli ( J ) and tubules ( K ) of DN mice ( n = 6). Scale bar = 10–50 μm. ( L ) Western blot analysis of SCAP, p-SREBP1, n-SREBP1, p-SREBP2, and n-SREBP2 in DN mouse renal cortex ( n = 6). ( M ) Representative immunohistochemical staining of F4/80 in kidneys of DN mice ( n = 6). Scale bar = 50 μm. ( N ) Relative mRNA levels of inflammatory cytokines (IL-1β, IL-6, TNF-α) in the DN mouse renal cortex ( n = 6). ( O ) Representative immunofluorescence staining of SCAP with cell markers (synaptopodin for <t>podocytes,</t> <t>PDGFRβ</t> for mesangial cells, <t>LTL</t> for proximal tubules) in DN mice. Scale bar = 10–20 μm. Data are represented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001
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    SCAP/SREBPs upregulation induces lipid deposition and inflammation in DN. ( A ) Multispecies conjoint analysis of conserved biological process in DN. ( B ) Schematic of SCAP/SREBPs as integrators of lipid metabolic and inflammatory signaling pathways. ( C , D ) Representative immunohistochemical staining for SREBP1, SREBP2, and SCAP in renal glomeruli ( C ) and tubules ( D ) of DN patients ( n = 6). Scale bars = 20–50 μm. ( E ) Representative immunohistochemical staining of CD68 in kidney of DN patients ( n = 6). Scale bar = 50 μm. ( F ) Heat map of Scap, Srebf1, Srebf2 and their downstream target gene expression in DN mice ( n = 6) from GSE200322 . ( G ) Correlation analysis between Scap, Srebf1, Srebf2 and renal inflammation, fibrosis, glomerular/tubular injury markers in DN mice ( n = 6) from GSE200322 . ( H ) Scheme of the STZ/HFD-induced T2DN mouse model. ( I ) Representative Oil Red O staining of renal glomeruli and tubules in DN mice ( n = 6). Scale bar = 10–50 μm. ( J , K ) Representative immunohistochemical staining for SREBP1, SREBP2, and SCAP in renal glomeruli ( J ) and tubules ( K ) of DN mice ( n = 6). Scale bar = 10–50 μm. ( L ) Western blot analysis of SCAP, p-SREBP1, n-SREBP1, p-SREBP2, and n-SREBP2 in DN mouse renal cortex ( n = 6). ( M ) Representative immunohistochemical staining of F4/80 in kidneys of DN mice ( n = 6). Scale bar = 50 μm. ( N ) Relative mRNA levels of inflammatory cytokines (IL-1β, IL-6, TNF-α) in the DN mouse renal cortex ( n = 6). ( O ) Representative immunofluorescence staining of SCAP with cell markers (synaptopodin for <t>podocytes,</t> <t>PDGFRβ</t> for mesangial cells, <t>LTL</t> for proximal tubules) in DN mice. Scale bar = 10–20 μm. Data are represented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001
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    Image Search Results


    SCAP/SREBPs upregulation induces lipid deposition and inflammation in DN. ( A ) Multispecies conjoint analysis of conserved biological process in DN. ( B ) Schematic of SCAP/SREBPs as integrators of lipid metabolic and inflammatory signaling pathways. ( C , D ) Representative immunohistochemical staining for SREBP1, SREBP2, and SCAP in renal glomeruli ( C ) and tubules ( D ) of DN patients ( n = 6). Scale bars = 20–50 μm. ( E ) Representative immunohistochemical staining of CD68 in kidney of DN patients ( n = 6). Scale bar = 50 μm. ( F ) Heat map of Scap, Srebf1, Srebf2 and their downstream target gene expression in DN mice ( n = 6) from GSE200322 . ( G ) Correlation analysis between Scap, Srebf1, Srebf2 and renal inflammation, fibrosis, glomerular/tubular injury markers in DN mice ( n = 6) from GSE200322 . ( H ) Scheme of the STZ/HFD-induced T2DN mouse model. ( I ) Representative Oil Red O staining of renal glomeruli and tubules in DN mice ( n = 6). Scale bar = 10–50 μm. ( J , K ) Representative immunohistochemical staining for SREBP1, SREBP2, and SCAP in renal glomeruli ( J ) and tubules ( K ) of DN mice ( n = 6). Scale bar = 10–50 μm. ( L ) Western blot analysis of SCAP, p-SREBP1, n-SREBP1, p-SREBP2, and n-SREBP2 in DN mouse renal cortex ( n = 6). ( M ) Representative immunohistochemical staining of F4/80 in kidneys of DN mice ( n = 6). Scale bar = 50 μm. ( N ) Relative mRNA levels of inflammatory cytokines (IL-1β, IL-6, TNF-α) in the DN mouse renal cortex ( n = 6). ( O ) Representative immunofluorescence staining of SCAP with cell markers (synaptopodin for podocytes, PDGFRβ for mesangial cells, LTL for proximal tubules) in DN mice. Scale bar = 10–20 μm. Data are represented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Journal of Nanobiotechnology

    Article Title: An oral nanocombinatorial agent exhibits pleiotropic improvement in diabetic nephropathy via modulation of the SCAP/SREBPs pathway

    doi: 10.1186/s12951-026-04301-9

    Figure Lengend Snippet: SCAP/SREBPs upregulation induces lipid deposition and inflammation in DN. ( A ) Multispecies conjoint analysis of conserved biological process in DN. ( B ) Schematic of SCAP/SREBPs as integrators of lipid metabolic and inflammatory signaling pathways. ( C , D ) Representative immunohistochemical staining for SREBP1, SREBP2, and SCAP in renal glomeruli ( C ) and tubules ( D ) of DN patients ( n = 6). Scale bars = 20–50 μm. ( E ) Representative immunohistochemical staining of CD68 in kidney of DN patients ( n = 6). Scale bar = 50 μm. ( F ) Heat map of Scap, Srebf1, Srebf2 and their downstream target gene expression in DN mice ( n = 6) from GSE200322 . ( G ) Correlation analysis between Scap, Srebf1, Srebf2 and renal inflammation, fibrosis, glomerular/tubular injury markers in DN mice ( n = 6) from GSE200322 . ( H ) Scheme of the STZ/HFD-induced T2DN mouse model. ( I ) Representative Oil Red O staining of renal glomeruli and tubules in DN mice ( n = 6). Scale bar = 10–50 μm. ( J , K ) Representative immunohistochemical staining for SREBP1, SREBP2, and SCAP in renal glomeruli ( J ) and tubules ( K ) of DN mice ( n = 6). Scale bar = 10–50 μm. ( L ) Western blot analysis of SCAP, p-SREBP1, n-SREBP1, p-SREBP2, and n-SREBP2 in DN mouse renal cortex ( n = 6). ( M ) Representative immunohistochemical staining of F4/80 in kidneys of DN mice ( n = 6). Scale bar = 50 μm. ( N ) Relative mRNA levels of inflammatory cytokines (IL-1β, IL-6, TNF-α) in the DN mouse renal cortex ( n = 6). ( O ) Representative immunofluorescence staining of SCAP with cell markers (synaptopodin for podocytes, PDGFRβ for mesangial cells, LTL for proximal tubules) in DN mice. Scale bar = 10–20 μm. Data are represented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: Anti-PDGFRβ antibody (Catalog No. 3169) was purchased from CST (MA, USA), anti-LTL antibody (Catalog No. FL-1321-2) was purchased from vector laboratories (San Francisco, USA), anti-synaptopodin (Catalog No. sc-515842) antibody was purchased from Santa Cruz (Texas, USA), anti-Zo-1 antibody (Catalog No. TA5145) was purchased from Abmart (Shanghai, China).

    Techniques: Protein-Protein interactions, Immunohistochemical staining, Staining, Targeted Gene Expression, Western Blot, Immunofluorescence

    Therapeutic efficacy of oral CLNPs in DN mice. ( A ) Scheme of DN model establishment and treatment protocol, mice were administered lycorine or CLNPs solution daily via oral gavage at a dose equivalent to 15 mg/kg of lycorine. ( B-D ) UACR (B), SCr (C), and BUN (D) in DN mice after oral administration with lycorine (15 mg/kg) or CLNPs (equivalent 15 mg/kg lycorine) ( n = 6). ( E-I ) Representative H&E, PAS, and Masson staining and quantitative analysis of kidney sections from DN mice after treatments ( n = 6). Scale bar = 10, 25–50 μm. ( J-L ) Representative IHC staining and quantification of SCAP in renal glomeruli (J, K) and tubules (J, L) of DN mice after treatments ( n = 6). Scale bar = 10–25 μm. ( M ) Representative immunofluorescence co-staining of SCAP, Golgin97 and PDGFRβ in renal glomeruli of DN mice after treatments ( n = 6). Scale bar = 10 μm. ( N ) Representative immunofluorescence co-staining of SCAP, Golgin97 and LTL in renal tubules of DN mice after treatments ( n = 6). Scale bar = 25 μm. Data are represented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Journal of Nanobiotechnology

    Article Title: An oral nanocombinatorial agent exhibits pleiotropic improvement in diabetic nephropathy via modulation of the SCAP/SREBPs pathway

    doi: 10.1186/s12951-026-04301-9

    Figure Lengend Snippet: Therapeutic efficacy of oral CLNPs in DN mice. ( A ) Scheme of DN model establishment and treatment protocol, mice were administered lycorine or CLNPs solution daily via oral gavage at a dose equivalent to 15 mg/kg of lycorine. ( B-D ) UACR (B), SCr (C), and BUN (D) in DN mice after oral administration with lycorine (15 mg/kg) or CLNPs (equivalent 15 mg/kg lycorine) ( n = 6). ( E-I ) Representative H&E, PAS, and Masson staining and quantitative analysis of kidney sections from DN mice after treatments ( n = 6). Scale bar = 10, 25–50 μm. ( J-L ) Representative IHC staining and quantification of SCAP in renal glomeruli (J, K) and tubules (J, L) of DN mice after treatments ( n = 6). Scale bar = 10–25 μm. ( M ) Representative immunofluorescence co-staining of SCAP, Golgin97 and PDGFRβ in renal glomeruli of DN mice after treatments ( n = 6). Scale bar = 10 μm. ( N ) Representative immunofluorescence co-staining of SCAP, Golgin97 and LTL in renal tubules of DN mice after treatments ( n = 6). Scale bar = 25 μm. Data are represented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: Anti-PDGFRβ antibody (Catalog No. 3169) was purchased from CST (MA, USA), anti-LTL antibody (Catalog No. FL-1321-2) was purchased from vector laboratories (San Francisco, USA), anti-synaptopodin (Catalog No. sc-515842) antibody was purchased from Santa Cruz (Texas, USA), anti-Zo-1 antibody (Catalog No. TA5145) was purchased from Abmart (Shanghai, China).

    Techniques: Drug discovery, Staining, Immunohistochemistry, Immunofluorescence